TRDMT1 (DNMT2) Protein(T352-30G)
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Description :Recombinant full-length human TRDMT1 (DNMT2) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :BC047733
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~67 kDa
Gene Aliases :DNMT2; DMNT2; PUMET; RNMT1; MHSAIIP
Scientific Background :TRDMT1 is responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. TRDMT1 also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, TRDMT1 is distinct and highly conserved in its function among various species. The indirect sequence recognition is also a feature of eukaryotic DNA methyltransferases, which may have arisen from a DNMT2-like RNA methyltransferase (1). DNMT2 is involved in retrotransposon silencing and telomere integrity in somatic cells (2).
References :
1. Goll, M. G. et.al: Methylation of tRNA(Asp) by the DNA methyltransferase homolog Dnmt2. Science 311: 395-397, 2006.
2. Phalke, S. et.al: Retrotransposon silencing and telomere integrity in somatic cells of Drosophila depends on the cytosine-5 methyltransferase DNMT2. Nature Genet. 41: 696-702, 2009.
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Research Areas :Apoptosis/Autophagy, Cancer, Cell Cycle, Neurobiology, Cancer, Neurobiology, Apoptosis/Autophagy, Cellular Stress