DNMT1 Protein(D351-31G)
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Description :Recombinant mouse DNMT1 (766-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Mouse
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :766-end
Genbank Number :BC053047
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~132 kDa
Gene Aliases :Dnmt; MCMT; Cxxc9; MTase; Met-1; Dnmt1o; m.MmuI; Met1; MommeD2
Scientific Background :DNMT1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities (1). DNMT1 enzyme is crucial for stable expression and function in vivo (2). DNMT1 is essential for maintenance of DNA methylation and the interaction of DNMT1 with the replication machinery is not strictly necessary for maintenance of DNA methylation, but improves its efficiency. DNMT1 is necessary and sufficient to maintain global methylation and aberrant CpG island methylation in human cancer cells.
References :
1. Yoder, J. A. et.al: New 5-prime regions of the murine and human genes for DNA (cytosine-5)-methyltransferase. J. Biol. Chem. 271: 31092-31097, 1996.
2. Tucker, K. L. et.al: Complementation of methylation deficiency in embryonic stem cells by a DNA methyltransferase minigene. Proc. Nat. Acad. Sci. 93: 12920-12925, 1996.
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Research Areas :Apoptosis/Autophagy, Cancer, Cell Cycle, Neurobiology, Cancer, Neurobiology, Apoptosis/Autophagy, Cell Cycle