Anti-phospho-ZAP70 (Tyr319)(Z02-65R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the ZAP70 protein phosphorylated at tyrosine 319
Cited Applications :WB
Cross Reactivity :Human, Mouse and Rat
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :The antiserum was produced against synthesized phosphopeptide derived from human ZAP-70 around the phosphorylation site of tyrosine 319 (S-P-YP-S-D).
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of extract from Jurkat cells using ZAP70 antibody (Lane 1 and 2) and Anti-phospho-ZAP70 (Tyr319) (Lane 3 and 4).
Scientific Background :ZAP70 is a non-receptor protein tyrosine kinase (part of the Syk/Zap70 family) that is involved in signaling by the T-cell antigen receptor (TCR). Ligation of the TCR/CD3 receptor in Jurkat T-cells induces phosphoprotein complexes which contain ZAP70 (1). TCR zeta chains are initially phosphorylated by p56Lck that lead to the recruitment of ZAP70 via its SH2 domain. ZAP70 in turn phosphorylates other proteins in the TCR-phosphoprotein complex. One of the natural substrates for ZAP70 is the zeta-chain dimer of the TCR/CD3 complex (2).
References :
1. Duplay, P. et al: p56lck interacts via its src homology 2 domain with the ZAP-70 kinase. J Exp Med. 1994 Apr 1;179(4):1163-72.
2. Chan, A C. et al: Differential expression of ZAP-70 and Syk protein tyrosine kinases, and the role of this family of protein tyrosine kinases in TCR signaling. J Immunol. 1994 May 15;152(10):4758-66.
3. Salomon AR, et al. (2003) Proc Natl Acad Sci U S A; 100(2): 443-448
4. Ku GM, et al. (2001) EMBO J; 20(3): 457-465.
5. Tang J, et al. (1999) Proc Natl Acad Sci U S A; 96(17): 9775-9780.
6. Zhao Q, et al. (1996) Mol Cell Biol; 16(12): 6765-6774. 7. Williams BL, et al. (1999) EMBO J; 18(7): 1832-1844.
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Research Areas :Cancer, Cytoplasmic Tyrosine Kinases, Inflammation, NfkB Pathway, Cancer, Inflammation, NfkB Pathway, Cytoplasmic Tyrosine Kinases