ZAK, Active(Z01-10G)

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Description :Recombinant full-length human ZAK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :Full Length

Genbank Number :NM_133646

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70^oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~82 kDa

Gene Aliases :AZK, MLT, MRK, MLK7, MLTK, mlklak, MAP3K20

Scientific Background :ZAK is a member of the MAPKKK family of signal transduction molecules and mediates gamma radiation signaling leading to cell cycle arrest. The activity of ZAK plays a role in cell cycle checkpoint regulation as well as being involved in regulating actin organization (1). Expression of kinase-dead ZAK in mouse fibroblasts disrupts actin stress fibers and causes morphologic changes. ZAK can activate JNK through MKK4/MKK7 and ERK5/p38-gamma via MKK3/MKK6. Expression of ZAK increases the population of cells in the G2/M phase of the cell cycle, whereas dominant-negative ZAK attenuated the G2 arrest caused by gamma radiation (2).

References :
1. Yang J-J, et al: Mixed lineage kinase ZAK utilizing MKK7 and not MKK4 to activate the c-Jun N-terminal kinase and playing a role in the cell arrest. Biochem. Biophys. Res. Commun. 297: 105-110, 2002.

2. Gross E. A, et al: MRK, a mixed lineage kinase-related molecule that plays a role in gamma-radiation-induced cell cycle arrest. J. Biol. Chem. 277: 13873-13882, 2002

Product Sheets (By Lot #) :

S084-3.pdf

X479-2.pdf

Research Areas :Apoptosis/Autophagy, Cancer, Cell Cycle, ERK/MAPK Pathway, JNK/SAPK Pathway, p38 Pathway, Ser/Thr Kinases, Cancer, ERK/MAPK Pathway, p38 Pathway, JNK/SAPK Pathway, Apoptosis/Autophagy, Cell Cycle, Ser/Thr Kinases