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Description :Full-length recombinant mouse WEE2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :BC052883
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :96 kDa
Gene Aliases :WEE1B
Scientific Background :WEE2, also known as WEE1B, is a dual-specificity kinases that uniquely function to phosphorylate specific Tyr/Thr residues on CDKs. It belongs to WEE1 family that also includes WEE1 homolog 1 (WEE1, also designated as somatic WEE1A) and PKMYT1 (previously known as MYT1). It is conserved from yeast to humans and has been shown to suppress CDK1 activity by phosphorylating its inhibitory sites. A key M phase-promoting factor (MPF) inhibitory kinase in oocytes and is required for maintaining meiotic arrest.
1. Leise W 3rd, et al: Multiple Cdk1 inhibitory kinases regulate the cell cycle during development. Dev Biol. 2002 Sep 1;249(1):156-73.
2. Liu C, et al: Ser 15 of WEE1B is a potential PKA phosphorylation target in G2/M transition in one-cell stage mouse embryos. Mol Med Rep. 7(6):1929-37, 2013.
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Research Areas :Cytoplasmic Tyrosine Kinases, Cell Cycle, Cancer