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WEE1 Protein(W01-35G)

WEE1 Protein(W01-35G)

  • $215.00


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Description :Recombinant human WEE1 (247-end) was expressed by E. coli cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:E.coli

Sequence :247-end

Genbank Number :NM_003390

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Western Blot

Molecular Weight :~115 kDa

Gene Aliases :WEE1hu

Scientific Background :WEE1 is a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases that catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase (1). WEE1 is highly expressed in testis. WEE1 kinase phosphorylated the p34 (CDC2) -cyclin B complex on tyr15 and inactivated the p34 (CDC2)-cyclin B kinase (2).

References :
1. Heald, R. et.al: Human wee1 maintains mitotic timing by protecting the nucleus from cytoplasmically activated cdc2 kinase. Cell 74: 463-474, 1993.

2. Parker, L. L. et.al: Inactivation of the p34cdc2-cyclin B complex by the human WEE1 tyrosine kinase. Science 257: 2155-2157, 1992.

Product Sheets (By Lot #) :

R003-1.pdf

J207-1.pdf

C2174-6.pdf

Research Areas :Cancer, Cell Cycle, Cytoplasmic Tyrosine Kinases, Cancer, Cell Cycle, Cytoplasmic Tyrosine Kinases