WDR48 Protein(W298-31G)
FOR BULK ORDER REQUESTS PLEASE CONTACT US
Description :Recombinant human WDR48 (2-580) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST
Expression System:Sf9 insect cells using baculovirus
Sequence :2-580
Genbank Number :NM_020839
Alternative Name(s) :USP1-associated factor 1, WD repeat endosomal protein, p80
Formulation :Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glyercol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :Several WD40-repeat (WDR) proteins interact with and regulate the activity of USPs. A subfamily of USPs constituting of USP1, USP12, and USP46 share a common WDR partner WDR48/ UAF1 that stimulates the activity of these USPs (1). USP1/WDR48 complex controls the cellular levels of monoubiquitinated FANCD2 DNA repair, a regulation pivotal for an intact Fanconi anemia pathway (2). WDR48 in complex with USP12 suppresses proliferation of tumor by enhancing stability of PHLPP1 (3).
References :1. Hanwen, Z. et al: Structural insights into the activation of USP46 by WDR48 and WDR20. Cell Discov. 5:34, 2019.
2. Cohn, M.A. et al: UAF1 is a subunit of multiple deubiquitinating enzyme complexes. J Biol Chem. 284(8):5343-5351, 2009.
3. Gangula, N.R. et al: WD repeat protein WDR48 in complex with deubiquitinase USP12 suppresses Akt-dependent cell survival signaling by stabilizing PH domain leucine-rich repeat protein phosphatase 1 (PHLPP1). J Biol Chem. 288(48):34545-54, 2013.
Specific Activity :Sample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Molecular Weight :Observed MW ~95 kDa. Calculated MW ~93 kDa.
Product Sheets (By Lot #) :