WNK3, Active (W04-11G)
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Description :Recombinant human WNK3 (1-571) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :1-571
Genbank Number :NM_001002838
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~91 kDa
Gene Aliases :PRKWNK3; FLJ30437; FLJ42662; KIAA1566
Scientific Background :WNK3 is a member of serine-threonine protein kinases, which was named the with-no-lysine family (1). WNK3 is expressed in several tissues including kidney and brain (1). WNK3 can regulate the activity of the electroneutral cation-coupled chloride cotransporters by activating NKCC1/2 and NCC and inhibiting KCCs (1). WNK3 kinase function is required for normal blood pressure homeostasis and the control of neuronal excitability (2). Also, overexpression of WNK3 increases the survival of Hela cells through caspase-3 pathway (3).
References :
1. Rinehart J., et.al: WNK3 kinase is a positive regulator of NKCC2 and NCC, renal cation-Cl-cotransporters required for normal blood pressure homeostasis. Proc Natl Acad Sci USA.; 102(46):16777-82, 2005.
2. Kahle KT., et.al: WNK3 modulates transport of Cl- in and out of cells: implications for control of cell volume and neuronal excitability. Proc Natl Acad Sci USA.; 102(46):16783-8, 2005.
3. Veríssimo F., et.al: Protein kinase WNK3 increases cell survival in a caspase-3-dependent pathway. Oncogene, 25 (30): 4172-82, 2006.
Product Sheets (By Lot #) :
Research Areas :AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Cytoplasmic Tyrosine Kinases, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Lipid Kinases, Neurobiology, p38 Pathway