WNK2, Active(W03-11G)
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Description :Recombinant human WNK2 (1-489) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :1-489
Genbank Number :NM_006648
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~100 kDa
Gene Aliases :KIAA1760; NY-CO-43; P/OKcl.13; PRKWNK2; SDCCAG43
Scientific Background :WNK2 is a serine/threonine kinase of the WNK (with no lysine (K)) protein kinase subfamily that also include WNK1, 3 and 4. The WNK kinases feature a cysteine residue in place of a lysine residue conserved in subdomain II of most kinases. WNK2 is predominantly expressed in brain, heart muscle, small intestine, and colon. As a tumor suppressor, WNK2 is involved in the modulation of growth factor-induced cancer cell proliferation through the MEK1/ERK1/2 pathway. WNK2 expression was found down-regulated epigenetically by promoter methylation in human gliomas.
References :
1. Veríssimo F, Jordan P. WNK kinases, a novel protein kinase subfamily in multi-cellular organisms. Oncogene. 2001 Sep 6;20(39):5562-9.
2. Moniz S. et al. Protein kinase WNK2 inhibits cell proliferation by negatively modulating the activation of MEK1/ERK1/2. Oncogene. 2007 Sep 6;26(41):6071-81.
3. Moniz S, et al. Loss of WNK2 expression by promoter gene methylation occurs in adult gliomas and triggers Rac1-mediated tumour cell invasiveness. Hum Mol Genet. 2013;22:84–95.
Product Sheets (By Lot #) :
Research Areas :Cancer, Cardiovascular Disease, Cell Cycle, Ser/Thr Kinases