WNK1, Active(W02-11G)
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Description :Recombinant human WNK1 (181-507) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :181-507
Genbank Number :NM_018979
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~67 kDa
Gene Aliases :HSAN2; HSN2; KDP; KIAA0344; MGC163339; MGC163341; p65; PRKWNK1; PSK
Scientific Background :WNK1 is a member of the WNK subfamily of serine/threonine protein kinases that is a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in WNK1 have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. WNK1 is a regulator of blood pressure and deficiency of this protein in mice lowers the blood pressure (1). WNK1 can regulate the Ca(2+) sensing and the subsequent Ca(2+)-dependent interactions mediated by synaptotagmin C2 domains and WNK1 exhibits additive CFTR inhibition (2).
References :
1. Zambrowicz, B. P. et.al: Wnk1 kinase deficiency lowers blood pressure in mice: a gene-trap screen to identify potential targets for therapeutic intervention. Proc. Nat. Acad. Sci. 100: 14109-14114, 2003.
2. Lee, B.-H. et.al: WNK1 phosphorylates synaptotagmin 2 and modulates its membrane binding. Molec. Cell 15: 741-751, 2004.
Product Sheets (By Lot #) :
Research Areas :Cancer, Cardiovascular Disease, Cell Cycle, Ser/Thr Kinases, Cancer, Cardiovascular Disease, Cell Cycle, Ser/Thr Kinases