Due to Easter holiday, all delivery service requests will not be processed on Friday, April 10, 2020. Regular delivery schedule will resume on Monday, April 13, 2019.
VEGF (121aa)(V810-40N)

VEGF (121aa)(V810-40N)

  • $171.00


FOR BULK ORDER REQUESTS PLEASE CONTACT US

Description :Recombinant Human VEGF (121aa) was expressed in E. coli cells.

Species :Human

Tag :

Expression System:E.coli

Sequence :

Genbank Number :P15693

Storage, Stability and Shipping :The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications :Cell Assay

Endotoxin Level :<1.0 EU/μg of recombinant protein as determined by the LAL method

Molecular Weight :14.3 kDa

Gene Aliases :Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF-121aa, Recombinant Human Vascular Endothelial Growth Factor 121AA (VEGFA)

Reconstitution Protocol :A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Scientific Background :VEGF is a heavily glycosylated, homodimeric protein. The human factor occurs in several molecular variants of 121, 162, 145, 148, 165, 183, 189, 206 amino acids, arising by alternative splicing of the mRNA. The splice forms of VEGF differ in biological properties such as the receptor types, which they recognize and their interaction with heparan sulfate proteoglycans. VEGF121 and VEGF165 are soluble secreted forms of the factor. The interaction of VEGF with heparin-like molecules of the extracellular matrix is required for efficient receptor binding. Protamine sulfate and suramin are capable of replacing the receptor-bound factor. This recombinant construct represents the isoform 121 of VEGFA.

Sample Cell Assay Data :The ED50 as determined by the dose-dependent proliferation of human umbilical vein endothelial cells (HUVEC) was found to be 10 ng/mL

Purity :Recombinant Human VEGF (121aa) resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250

Product Sheets (By Lot #) :

G3223-25.pdf

Research Areas :Cancer