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Description :Chicken Polyclonal Antibody

Species :Chicken

Tag :

Expression System:

Sequence :

Specificity :Recognizes the Vimentin protein

Cited Applications :WB, IF

Cross Reactivity :Human, Mouse and Rat

Host :Chicken, IgY

Immunogen :Recombinant human vimentin purified from E. coli

Formulation :Total IgY fraction in PBS + 10 mM NaN3.

Purification :Total IgY fraction

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Western blot of NIH 3T3 cells showing specific immunolabeling of the ~50k Vimentin protein.

Sample Data :Mixed neuron/glial cultures stained with anti-Vimentin (green) and rabbit anti-GFAP antibody (catalog #620-GFAP) (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and Vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and Vimentin, the two proteins assemble to produce heteropolymer filaments.

Scientific Background :Vimentin is a member of intermediate filament family of proteins and is an important structural protein in eukaryotic cells (1). Vimentin is a component of the cytoskeleton long with microtubules and actin microfilaments. Studies have shown that Vimentin is associated with the nucleus, endoplasmic reticulum and mitochondria, either laterally or terminally. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol (2). Thus, Vimentin plays a key role in maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. Copolymers are frequently formed between Vimentin and other intermediate filaments, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Thus, antibodies to Vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that Vimentin plays a part in prostate cancer cell motility and invasiveness (3).

References :
1. Fuchs, E., et al: Intermediate filaments: structure, dynamics, function, and disease". Annu Rev Biochem,1994; 63: pp. 345–82.

2. Katsumoto, T. et al: The role of the vimentin intermediate filaments in rat 3Y1 cells elucidated by immunoelectron microscopy and computer-graphic reconstruction. Biol Cell,1990; 68 (2): pp. 139–46.

3. Zhao Y. et al: Vimentin affects the mobility and invasiveness of prostate cancer cells. Cell Biochem Funct. 2008 Sep-Oct;26(5):571-7.

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Research Areas :Neurobiology