Anti-VAV(V14-63R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the VAV protein
Cited Applications :WB, IP
Cross Reactivity :Western blot VAV from human cells
VAV from other species may also be detectable.
Host :Rabbit
Immunogen :Synthetic peptide corresponding to amino acids 172-176 of human VAV
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative Immunoprecipitation with 2 μg of Anti-VAV using 500 μg of Jurkat and THP1cell lysates.(IP)gel.jpg)
Sample Data :Representative Western blot with Anti-VAV (1:1000) using 3 μg, 10 μg and 30 μg of Jurkat cell lysate.(WB)gel.jpg)
Scientific Background :VAV is a proto-oncogene that is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. VAV plays a vital role in hematopoiesis and in T-cell and B-cell development as well as in their activation. VAV is the specific binding partner of Nef proteins from HIV-1 (1). Co-expression and binding of these VAV partners initiates profound morphological changes, cytoskeletal rearrangements and JNK/SAPK pathway activation leading to increased levels of viral transcription and replication. VAV is also the guanine nucleotide dissociation inhibitor for Ras.
References :
1. Fackler, O T. et al: Activation of Vav by Nef induces cytoskeletal rearrangements and downstream effector functions. Molec. Cell 3729-739, 1999.
Product Sheets (By Lot #) :
Research Areas :Apoptosis/Autophagy, ERK/MAPK Pathway, JNK/SAPK Pathway, p38 Pathway, ERK/MAPK Pathway, p38 Pathway, JNK/SAPK Pathway, Apoptosis/Autophagy