Anti-VLK(V05-163R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the VLK protein
Cited Applications :WB, ELISA, IF, IHC
Cross Reactivity :Human, Mouse and Rat
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :VLK antibody was raised against a 15 amino acid synthetic peptide from near the center of human VLK
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of VLK in human lung tissue lysate with VLK antibody at 1 ug/ml.
Sample Data :Immunohistochemistry of VLK in rat lung tissue with VLK antibody at 2.5 ug/ml.
Scientific Background :VLK or vertebrate lonesome kinase is a protein kinase which is first expressed in E-cadherin-positive anterior visceral endoderm and mesendoderm, and later its expression is confined to E-cadherin-negative mesenchyme. VLK regulates the rate of protein export from the Golgi and thereby plays an important role in the formation of functional stroma by mesenchymal cells (1). Targeted disruption of VLK leads to a defect in lung development and delayed ossification of endochondral bone. VLK deficient mice display neonatal lethality due to respiratory failure, with a suckling defect arising from a cleft palate. VLK is required for the appropriate timing of flat proliferative chondrocyte differentiation (2).
References :
1. Kinoshita. et.al: The novel protein kinase Vlk is essential for stromal function of mesenchymal cells.Development 136: 2069-2079, 2009.
2. Imuta, Y. et al: Short limbs, cleft palate, and delayed formation of flat proliferative chondrocytes in mice with targeted disruption of a putative protein kinase gene, Pkdcc (AW548124). Dev Dyn. 2009 Jan; 238(1):210-22.
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Research Areas :Cancer, Cytoplasmic Tyrosine Kinases, Invasion/Metastasis, Neurobiology, Ser/Thr Kinases, Cancer, Neurobiology, Invasion/Metastasis, Ser/Thr Kinases, Cytoplasmic Tyrosine Kinases