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USP28, Active (U528-381G)

USP28, Active (U528-381G)

  • $226.00


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Description :  Recombinant human USP28 (155-675a.a.) was expressed in E. coli cells using an N-terminal GST tag.

Species : Human

Tag : GST tag

Expression System : E.coli

Sequence : 155-675a.a.

Genbank Number : NM_020886

Specific Activity : Sample Activity Plot. For specific information on a given lot, see related technical data sheet. 

Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet. 

Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications : Activity Assay, Western Blot

Molecular Weight :  85 kDa

Gene Aliases :  KIAA1515

Scientific Background : USP28 (ubiquitin carboxyl-terminal hydrolase 28) is a deubiquitinase belonging to the family of deubiquitinating enzymes (DUBs) and plays a role in the DNA damage pathway by specifically deubiquitinating proteins of the pathway such as CLSPN. USP2 deubiquitinates ZNF304, inhibiting its degradation and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in various colorectal cancers (CRC) cells (1). USP28 counteracts the activity of E3 ligases, antagonizes FBW7 and a component of the SCF-type E3 ubiquitin ligase that targets key transcriptional factors to ubiquitin-directed proteasome degradation, thus stabilizing substrates such as oncogenic c-MYC (2). This may lead to enhanced proliferation, carcinogenesis, and metastasis.

References : 
1. Serra, Ryan W et al: A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype. eLife 3: e02313, 2014.

2. Wang, X. et al: Targeting deubiquitinase USP28 for cancer therapy. Cell Death & Disease 9: 186, 2018.

Product Sheets: (by Lot #):

D2352-6.pdf

Research Areas : Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Invasion/Metastasis, Metabolic Disorder