HOLIDAY SHIPPING UPDATE: All delivery service requests will not be processed on Friday April 2, 2021 as it is Good Friday holiday. Regular delivery schedule will resume the next business day.
USP25, Protein (U525-31G)

USP25, Protein (U525-31G)

  • $215.00


FOR BULK ORDER REQUESTS PLEASE CONTACT US

Description : Recombinant human USP25 (162-677 a.a.) was expressed in E. coli cells using an N-terminal GST tag.

Species : Human

Tag : GST tag

Expression System : E. coli cells

Sequence : 162-677 a.a.

Genbank Number : NM_013396

Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
USP25

Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications : Western Blot

Molecular Weight :  85 kDa

Gene Aliases : USP21

Scientific Background : Ubiquitin carboxyl-terminal hydrolase 25 (USP25) belongs to the ubiquitin-specific proteases (USPs) family, and removes conjugated ubiquitin from substrates (1). USP25 processes, recycles ubiquitin to prevent the proteasomal degradation of proteins (1). It modifies tetraubiquitin chains via hydrolysing ‘Lys-63’ linked polyubiquitins to negatively regulate the inflammation mediated by IL-17 (2). A genome-wide association study of inflammatory bowel disease (IBD) identified the SNPs at USP25 sites (3).

References : 
1. Valero R, et al. USP25, a novel gene encoding a deubiquitinating enzyme, is located in the gene-poor region 21q11.2. Genomics. 1999 Dec 15;62(3):395-405.

2. Zhong B, et al. Negative regulation of IL-17-mediated signaling and inflammation by the ubiquitin-specific protease USP25. Nat Immunol. 2012 Nov; 13(11):1110-7.

3. Brant SR, et al. Genome-Wide Association Study Identifies African-Specific Susceptibility Loci in African Americans With Inflammatory Bowel Disease. Gastroenterology. 2017 Jan; 152 (1):206-217.

Product Sheets: (by Lot #):

D2279-4a.pdf

Research Areas : Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Invasion/Metastasis, Metabolic Disorder