UBE2K (UBC1), Active(U227-380H)
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Description :Recombinant full-length human UBE2K was expressed in E. coli cells using an N-terminal His tag.
Tag :His tag
Sequence :Full length
Genbank Number :NM_005339
Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Ubiquitin Enzyme Assay, Western Blot
Molecular Weight :26 kDa
Gene Aliases :E2-25K; HIP2; HYPG; LIG; UBC1
Scientific Background :UBE2K also known as UBC1 or ubiquitin-conjugating enzyme E2K belongs to the ubiquitin-conjugating enzyme family which interacts with RING finger proteins. UBE2K can ubiquitinate huntingtin, the gene product for Huntington's disease. Huntingtin is ubiquitinated in peripheral cells, suggesting a role for UBE2K in the degradation of huntingtin. UBE2K plays a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases. UBE2K also plays a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation.
1. Kalchman, M.A. et al: Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme. J. Biol. Chem. 271: 19385-19394, 1996
2. De Pril, R. et al: Ubiquitin-conjugating enzyme E2-25K increases aggregate formation and cell death in polyglutamine diseases. Mol Cell Neurosci. 2007 Jan;34(1):10-9
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Research Areas :Cancer, Cell Cycle, Neurobiology