UBE2R2 (UBC3B) Protein(U235-30H)

UBE2R2 (UBC3B) Protein(U235-30H)

  • $105.00


Description :Recombinant human UBE2R2 (UBC3B) (2-end) was expressed in E. coli cells using an N-terminal His tag.

Species :Human

Tag :HIS tag

Expression System:E.coli

Sequence :2-end

Genbank Number :NM_017811

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Western Blot

Molecular Weight :~32 kDa

Gene Aliases :CDC34B; E2-CDC34B; UBC3B

Scientific Background :UBE2R2 (UBC3B) or ubiquitin-conjugating enzyme E2R 2 encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP therby enhancing beta-catenin degradation. CK2-dependent phosphorylation of CDC34 and UBC3B functions by regulating BTRC substrate recognition (1). UBE2R2 complements a yeast cdc34 temperature-sensitive mutant. Deletion and site-directed mutagenesis demonstrated that CK2 phosphorylated UBE2R2 in the C-terminal domain at serine-233; mutagenesis of the corresponding serine residue at position 231 of human CDC34/UBC3 showed that this was the major CK2-phosphorylation site in this protein.

References :
1. Semplici, F. et.al: CK2-dependent phosphorylation of the E2 ubiquitin conjugating enzyme UBC3B induces its interaction with beta-TrCP and enhances beta-catenin degradation. Oncogene 21: 3978-3987, 2002.

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Research Areas :Neurobiology, Cell Cycle, Cancer