Anti-Phospho-UBLE1A (SAE1) (Ser185)(U208-65R)
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Description :Rabbit Polyclonal Antibody
Species :
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Expression System:
Sequence :
Specificity :Recognizes the human SUMO Activating Enzyme E1 protein
Cited Applications :ELISA, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human, Bovine, Mouse, Rat and Dog
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against synthesized peptide corresponding to a region surrounding Serine185 of the human SUMO Activating Enzyme E1 protein
Purification :Affinity chromatography
Stability :1yr at –20oC from date of shipment.
Sample Data :Western blot using Anti-Phospho-UBlE1A (SAE1) (Ser185) antibody (2 µg/mL). Left lane (-) contains 20 µg human HeLa whole cell protein. Right lane (+) contains 20 µg human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephos-phorylation of the target.
Scientific Background :UBLE1A (also known as SAE1) or SUMO1 activating enzyme subunit 1 is involved in regulating protein structure and intracellular localization. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (1). The SAE1/SAE2 dimer functions in SUMO1 activation in a manner analogous to the single E1 ubiquitin-activating enzymes. The SAE2 inactivation may be a therapeutic strategy in MYC-driven cancers (2).
References :
1. Okuma, T. et.al: In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2. Biochem. Biophys. Res. Commun. 254: 693-698, 1999.
2. Kessler, J. D. et.al: A SUMOylation-dependent transcriptional subprogram is required for Myc-driven tumorigenesis. Science 369: 348-353, 2012.
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Research Areas :Angiogenesis, Apoptosis/Autophagy, Cancer, Cell Cycle, Cellular Stress, Inflammation, Invasion/Metastasis, Neurobiology