Anti-UBA1 (UBE1)(U201-62R)

Anti-UBA1 (UBE1)(U201-62R)

  • $675.00


Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the human Ubiquitin Activating Enzyme E1 protein

Cited Applications :ELISA, IHC, WB

Cross Reactivity :Human

Host Isotype / Clone# :Rabbit, IgG

Immunogen :The antibody was produced against recombinant protein corresponding to full-length human Ubiquitin Activating Enzyme E1

Purification :Multistep procedure

Stability :1yr at –20oC from date of shipment

Sample Data :Immunohistochemical analysis of formalin-fixed, paraffin embedded human lung tissue using Anti-UBA1 (UBE1) (10 µg/ml)human lung tissue.

Sample Data :Western blot using Anti-UBA1 (UBE1) (1:1,000 dilution) shows detection of a band at ~118 kDa corresponding to UBE1 (lane 1 800 nm channel). Load: ~35 µg of an A431 whole cell lysate.

Scientific Background :UBA1 or ubiquitin-like modifier activating enzyme 1 is an E1 enzyme that initiates the activation and conjugation of ubiquitin-like proteins and catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation (1). UBE1 is able to transfer ubiquitin to a wide range of E2 substrates. UBA1 protein may participate in DNA topoisomerase-2 activity (2). UBA1 complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair.

References :
1. Jin, Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Nature 447: 1135-1138, 2007.

2. Zacksenhaus, Identification of human gene complementing ts A1S9 mouse L-cell defect in DNA replication following DNA-mediated gene transfer. Somat. Cell Molec. Genet. 14: 371-379, 1988.

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Research Areas :Cancer, Cell Cycle, Neurobiology