• $455.00


Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the UBC9 protein

Cited Applications :WB

Cross Reactivity :Western blot UBC9 from human and mouse cells

UBC9 from other species may also be detectable.

Host :Rabbit

Immunogen :Synthetic peptide of c-terminal amino acid sequence of human UBC9 (P63279)

Purification :Affinity chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Representative western blot with Anti-UBC9 (1:1000) using 3 μg, 10 μg and 30 μg of Jurkat cell lysate.

Scientific Background :UBC9 is a member of the E2 ubiquitin-conjugating enzyme family that is involved in ubiquitin-dependent protein degradation system (1). Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes and ubiquitin-protein ligases. UBC9 can function as a co-factor of PLAGL2, uncoupling its enzymatic activity to mediate PLAGL2 interactive SP-C promoter activity (2). UBC9 also plays an important role in the up-regulation of the bone morphogenetic protein signaling pathway.

References :
1. Yasugi, T. Identification of the structural and functional human homolog of the yeast ubiquitin conjugating enzyme UBC9. Nucleic Acids Res. 24: 2005-2010, 1996.

2. Wang, Z.-Y. Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9. J. Biol. Chem. 271: 24811-24816, 1996.

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Research Areas :Cancer, Neurobiology, Cancer, Neurobiology