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TOPK, Unactive(T14-14G)

TOPK, Unactive(T14-14G)

  • $215.00


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Description :Recombinant full-length human TOPK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :Full Length

Genbank Number :NM_018492

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~68 kDa

Gene Aliases :PBK, SPK, Nori-3, FLJ14385

Scientific Background :TOPK is a MAPK kinase that phosphorylates p38 MAPK and is activated in a cell-cycle-dependent manner in neuronal progenitor cells in vitro (1). Expression of TOPK is detected in male germ line progenitor cells, activated T-cells, and a variety of lymphomas and leukemias. In vitro studies have shown that activated TOPK phosphorylated p38 MAPK but not JNK or ERK. TOPK activation requires phosphorylation by both the M-phase CDK1/CyclinB kinase complex and another unknown kinase, possibly Raf C or Raf A. TOPK may play an important role in linking extracellular signals to an intracellular state, possibly allowing extracellular influence on the cell-cycle-related processes of proliferation or differentiation (2).

References :
1. Matsumoto, S. et al: Characterization of a MAPKK-like protein kinase TOPK. Biochem Biophys Res Commun. 2004; 325: 997-1004.

2. Simons-Evelyn, M. et al: PBK/TOPK is a novel mitotic kinase which is upregulated in Burkitt's lymphoma and other highly proliferative malignant cells. Blood Cells Mol Dis, 2001; 27: 825-829.

Product Sheets (By Lot #) :

N319-1.pdf

Research Areas :Cancer, Ser/Thr Kinases, Cancer, Ser/Thr Kinases