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  • $193.00


Description :Recombinant Human TNF was expressed in E. coli cells.

Species :Human

Tag :

Expression System:E.coli

Sequence :

Genbank Number :P01375

Storage, Stability and Shipping :The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications :Cell Assay

Endotoxin Level :<1.0 EU/μg of recombinant protein as determined by the LAL method

Molecular Weight :17.6 kDa

Gene Aliases :TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin, Recombinant Human Tumor Necrosis Factor Alpha (TNF)

Reconstitution Protocol :A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Scientific Background :Tumor Necrosis Factor is secreted by macrophages, monocytes, neutrophils, T cells, natural killer cells following their stimulation by bacterial lipopolysaccharides although cells expressing CD8(+) secrete little or no TNF-alpha. In addition, TNF is secreted by peripheral neutrophilic granulocytes and by a number of transformed cell lines that include those of astrocytes, microglial cells, smooth muscle cells and fibroblasts. Human milk also contains this factor. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, GM-CSF, SP, Bradykinin, Immune complexes, inhibitors of cyclooxygenase and platelet activating factor. TNF-alpha shares approximately 30% homology with TNF-beta. Human TNF-alpha is a non-glycosylated protein of 17.6 kDa.

Sample Cell Assay Data :The ED50 as determined by dose dependent proliferation of IL4 stimulated human megakaryocytic cell line was found to be ≤0.5 ng/mL

Purity :Recombinant Human TNF resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250

Product Sheets (By Lot #) :


Research Areas :Cancer