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Description :Recombinant tag-free human thrombin (364-end) was expressed in E. coli cells.
Sample Data :
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Enzyme Commission Number :EC 126.96.36.199
Gene Aliases :F2; PT; RPRGL2; THPH; coagulation Factor II; Factor IIa
Storage, Stability, and Shipping :Store product at –20oC for up to 1 year. Aliquot enzymes to avoid freeze / thaw cycles.
Applications :In vitro Proteolysis
Digestion Conditions :Catalytic pH range: 5 ~ 10
Catalytic temperature range: 20 ~ 37oC
Catalytic time range: 0.3 ~ 16h
Scientific Background :Thrombin is an endolytic serine protease that cleaves fibrinogen to produce insoluble active fibrin strands and fibrinopeptides A and B. This enzymatic process is essential for blood clot formation. Thrombin recognizes the consensus sequence A-B-Pro-Arg-▼-X-Y, with the first two amino acids representing hydrophobic residues and the last two amino acids representing non-acidic residues. The common recognized sequences are Leu-Val-Pro-Arg-▼-Gly-Ser and Gly-Arg-▼-Gly. Thrombin has high proteolytic specificity and hydrolytic efficiency, making it a widely used tool for cleaving purification tag from recombinant fusion proteins.
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