TFG-ALK (Tex4Aex20), Active (A19-19KG)

TFG-ALK (Tex4Aex20), Active (A19-19KG)

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Description :Recombinant human fusion protein TFG (1-138a.a., exons1-4)-ALK (1058a.a.-end, exons20-29) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence : 1-138a.a., exons1-4 (TFG) - 1058a.a.-end, exons20-29 (ALK)

Genbank Number 1:NM_001195478

Genbank Number 2:NM_004304

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay

Molecular Weight :106 kDa

Gene Aliases :TFG: HMSNP; SPG57; TF6
ALK: ALK (Ki-1), CD246

Scientific Background : Anaplastic large cell lymphomas are associated with chromosomal aberrations involving the anaplastic lymphoma kinase (ALK) gene, resulting in the expression of novel chimeric ALK proteins with transforming properties. In most of these tumors, the t(2;5)(p23;q35) generates the NPM-ALK fusion gene. Genes other than NPM have been demonstrated to fuse to the ALK gene. Two different ALK rearrangements involving the TRK-fused gene (TFG) in which the same portion of ALK was fused to different length fragments of the 5' TFG region. These two rearrangements encoded chimeric proteins of 85 kd (TFG-ALK(S)) and 97 kd (TFG-ALK(L)), respectively. TFG can provide an alternative to NPM as a fusion partner responsible for activation of the ALK and the pathogenesis of ALCL.

References :
1. Hernández L. et al: TRK-fused gene (TFG) is a new partner of ALK in anaplastic large cell lymphoma producing two structurally different TFG-ALK translocations. Blood. 1999 Nov 1;94(9):3265-8.

2. Hernández L. et al: Diversity of genomic breakpoints in TFG-ALK translocations in anaplastic large cell lymphomas: identification of a new TFG-ALK(XL) chimeric gene with transforming activity. Am J Pathol. 2002 160(4):1487-94. 2002.

Product Sheets (By Lot #) :

M2720-6.pdf

Research Areas :AKT/PKB Pathway, Cancer, Cardiovascular Disease, JAK/STAT Pathway, Neurobiology, Ser/Thr Kinases