Thrombin, Active(T565-31N)

Thrombin, Active(T565-31N)

  • $325.00


Description :Recombinant tag-free human thrombin (364-end) was expressed in E. coli cells.

Species :Human

Tag :tag-free

Expression System:E.coli

Sequence :364-end

Sample Data :

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Enzyme Commission Number :EC

Gene Aliases :F2; PT; RPRGL2; THPH; coagulation Factor II; Factor IIa

Storage, Stability, and Shipping :Store product at –20oC for up to 1 year. Aliquot enzymes to avoid freeze / thaw cycles.

Applications :In vitro Proteolysis

Digestion Conditions :Catalytic pH range: 5 ~ 10
Catalytic temperature range: 20 ~ 37oC
Catalytic time range: 0.3 ~ 16h

Scientific Background :Thrombin is an endolytic serine protease that cleaves fibrinogen to produce insoluble active fibrin strands and fibrinopeptides A and B. This enzymatic process is essential for blood clot formation. Thrombin recognizes the consensus sequence A-B-Pro-Arg-▼-X-Y, with the first two amino acids representing hydrophobic residues and the last two amino acids representing non-acidic residues. The common recognized sequences are Leu-Val-Pro-Arg-▼-Gly-Ser and Gly-Arg-▼-Gly. Thrombin has high proteolytic specificity and hydrolytic efficiency, making it a widely used tool for cleaving purification tag  from recombinant fusion proteins.

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