TEV (Tobacco Etch Virus) Protease, Active(T525-31H)
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Description :Recombinant TEV (Tobacco Etch Virus) protease (2038-2279aa) was expressed in E. coli cells using an N-terminal His tag.
Species :TE Virus
Tag :His tag
Expression System:E.coli
Sequence :2038-2279 aa
Sample Data :
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Enzyme Commission Number :EC 3.4.22.44
Gene Aliases :NI-a, NIa-pro, 49kDa-Pro
Storage, Stability, and Shipping :Store product at –70oC for up to 1 year. Aliquot enzymes to avoid freeze / thaw cycles.
Applications :In vitro Proteolysis
Digestion Conditions :Catalytic pH range: 5.5 ~ 8.5; catalytic temperature range: 4 ~ 30oC
Scientific Background :Recombinant Tobacco Etch Virus (TEV) Protease is derived from the naturally occurring 28 kDa Nla recombinant protease. TEV Protease is commonly used for removing affinity tags from purified recombinant fusion proteins. TEV protease is highly site-specific, targeting the consensus sequence EXXYXQ(G/S). Cleavage occurs between Q and G/S. The most common TEV target sequences are ENLYFQG or ENLYFQS. The enzyme retains activity in solutions containing 1M Urea, 1-5mM DTT, beta-Mercaptoethanol, EDTA and PMSF, Aprotinin, Leupeptin and other protease inhibitors. The 6-His tag enables easy removal of the protease.
Product Sheets (By Lot #) :