T4 gp32 Protein (T4G32-C501H)

T4 gp32 Protein (T4G32-C501H)

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Description : Recombinant full-length T4 gp32 Protein from Escherichia virus T4 was expressed in E. coli using N-terminal His tag.

Species : Escherichia virus

Tag : HIS tag

Expression System: E.coli

Genbank Number : J02513.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

 

Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight : 38 kDa

Gene Aliases : ss-DNA binding protein (g32), SSB protein, Helix-destabilizing protein

Scientific Background : Bacteriophage T4 gene 32 protein (gp32) is a single-stranded DNA binding protein with key role in DNA replication, recombination, and repair. T4 gp32 is a zinc metalloprotein with three functional domains: the N-terminal basic domain, the core domain and the C-terminal acidic domain. The ssDNA binding site lies in the core domain. In vitro, T4 gp32 binds cooperatively to single stranded DNA and stimulates cell-free DNA synthesis directed by the T4-encoded DNA polymerase (1,2)

References :

  1. Jordan, C.S. et al: Regulation of the bacteriophage T4 Dda helicase by Gp32 single-stranded DNA-binding protein. DNA Repair (Amst), 2015; 25: 41-53.
  2. Pant, K. et al: The role of the C-domain of bacteriophage T4 gene 32 protein in ssDNA binding and dsDNA helix-destabilization: Kinetic, single-molecule, and cross-linking studies. PLoS One. 2018; 10:13(4):e0194357.

Product Sheets (By Lot #) :

J4005-7