TNIK, Active(T27-11G)
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Description :Recombinant human TNIK (1-367) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :1-367
Genbank Number :NM_015028
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~67 kDa
Gene Aliases :(None)
Scientific Background :TNIK or TRAF2 and NCK interacting kinase is characterized by an N-terminal kinase domain and a C-terminal GCK domain that serves a regulatory function (1). TNIK is mainly expression in brain, heart, and spleen and it is a specific effector of RAP2 which regulate actin cytoskeleton (2). TNIK is autophosphorylated in a manner dependent upon lys54 in the ATP-binding pocket of its kinase domain and plays a main role in cytoskeleton regulation.
References :
1. Fu, C. A. et.al: TNIK, a novel member of the germinal center kinase family that activates the c-Jun N-terminal kinase pathway and regulates the cytoskeleton. J. Biol. Chem. 274: 30729-30737, 1999.
2. Taira, K. et.al: The Traf2- and Nck-interacting kinase as a putative effector of Rap2 to regulate actin cytoskeleton. J. Biol. Chem. 279: 49488-49496, 2004.
Product Sheets (By Lot #) :
Research Areas :Cancer, Inflammation, Neurobiology, NfkB Pathway, Ser/Thr Kinases, WNT Signaling, Cancer, Neurobiology, Inflammation, NfkB Pathway, WNT Signaling, Ser/Thr Kinases