TRIO, Active(T16-11G)

TRIO, Active(T16-11G)

  • $226.00


Description :Recombinant human TRIO (2627-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :2627-end

Genbank Number :O75962-1

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~82 kDa

Gene Aliases :(None)

Scientific Background :TRIO (Triple functional domain protein) belongs to CAMK Ser/Thr protein kinase family with 5 isoforms produced by alternative splicing. It encodes two Dbl-homology-Pleckstrin-homology (DH-PH) Rho-GEF units with different specificities and a C-terminal serine/threonine kinase domain. It is a Rho-guanine nucleotide exchange factors (GEFs) that catalyzes exchange of GDP for GTP on small Rho-GTPases, such as Rac1, RhoG and RhoA. TRIO was originally identified as a binding partner of the transmembrane tyrosine phosphatase LAR, and they together play a role in coordinating cell-matrix and cytoskeletal rearrangements required for cell migration and cell growth.

References :
1. Debant A, et al. The multidomain protein Trio binds the LAR transmembrane tyrosine phosphatase, contains a protein kinase domain, and has separate rac-specific and rho-specific guanine nucleotide exchange factor domains. Proc Natl Acad Sci U S A. 93:5466-71. 1996.

2. van Rijssel J, et al: The many faces of the guanine-nucleotide exchange factor trio. Cell Adh Migr. 6:482-7. 2012.

Product Sheets (By Lot #) :


Research Areas :Neurobiology, ERK/MAPK Pathway, Cancer, Ser/Thr Kinases