TLK1, Active(T10-10G)
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Description :Recombinant full-length human TLK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_012290
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~135 kDa
Gene Aliases :KIAA0137; PKU-beta
Scientific Background :TLK1 or tousled-like kinase 1 is a nuclear serine/threonine kinase that is potentially involved in the regulation of chromatin assembly. TLK1 is first described in Arabidopsis. TLK1 is a novel target of the DNA damage checkpoint that is rapidly inactivated upon exposure to ionizing radiation (IR) and the inactivation is directly mediated by the S-phase DNA damage checkpoint (1). The suppression of TLK1 activity after double-strand breaks in the DNA, replication blockade, or low doses of ultraviolet irradiation requires ATM, NBS and CHK1 (2).
References :
1. Groth, A. et.al: Human Tousled like kinases are targeted by an ATM- and Chk1-dependent DNA damage checkpoint. EMBO J. 22: 1676-1687, 2003.
2. Krause, D. R. et.al: Suppression of Tousled-like kinase activity after DNA damage or replication block requires ATM, NBS and Chk1. Oncogene 22: 5927-5937, 2003.
Product Sheets (By Lot #) :
Research Areas :Ser/Thr Kinases, Neurobiology, Inflammation, Cardiovascular Disease, Cancer, Angiogenesis, Cancer, Neurobiology, Inflammation, Cardiovascular Disease, Angiogenesis, Ser/Thr Kinases