Tau Antibody (pSer202/pThr205), AH36(T08-65R)

Tau Antibody (pSer202/pThr205), AH36(T08-65R)

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Description :Rabbit Monoclonal Antibody

 

Genbank Number :NM_020630

Cited Applications :WB (1:500); ICC/IF (1:500); 1HC (1:500); DB; ELISA

Ideal working dilutions for each application should be empirically determined by the investigator.

Specificity :Detects ~79 kDa.

Cross Reactivity :Human, Mouse

Host/Isotype/Clone# :Rabbit / IgG / AH36

Immunogen :Synthetic peptide of Human Phospho Tau (Ser202/Thr205)

Formulation :PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.

Stability :1yr at –200C from date of shipment. Avoid freeze/thaw cycle.

Scientific Background :Alzheimer’s Disease (AD) is the most common neurodegenerative disease, affecting 10% of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3). Phosphorylation at serine 202 and threonine 205 induces a turn-like structure and drives tau aggregation (4). T08-65R recognizes the same epitope as the AT8 phospho tau antibody.

References :1. www.alz.org/alzheimers-dementia/facts-figures
2. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146–148 (1907)
3. Matsumoto, G. et al. (2018). Int J Mol Sci. 19, 1497.
4. Depres, C. et al. (2017). PNAS 114(34):9080-9085.

Sample Data :Dot Blot analysis using Tau Monoclonal Antibody, AH36 (Cat# T08-65R). Species: E. Coli, Baculovirus. Primary Antibody: Rabbit Tau Monoclonal Antibody (Cat# T08-65R) at 1:500. Secondary Antibody: Goat Anti-Rabbit IgG:HRP.

Sample Data :Western Blot analysis of Human iPSC-derived cortical neurons showing detection of Tau protein using Rabbit Tau Monoclonal Antibody, AH36 (Cat# T08-65R). Lane 1: MW ladder. Lane 2: Control (non-disease) line. Lane 2: Ex10+16 tau mutant sample. Lane 3: P301L Tau mutant sample. The bar graph on the right shows the quantification of pSer202/pThr205 compared to total tau in each sample.

Product Sheets (By Lot #) :

E4198-17.pdf

Research Areas :Neurobiology