Tau Antibody(T08-63R)
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Description :Rabbit Polyclonal Antibody
Genbank Number :NM_020630
Cited Applications : WB (1:1000)
Ideal working dilutions for each application should be empirically determined by the investigator
Specificity :Detects ~10.2 kDa.
Cross Reactivity :Human, Mouse, Rat
Cross Reactivity :Human, Mouse, Rat
Host :Rabbit
Immunogen :Recombinant Human Tau AA297-391 (dGAE) Fibril (StressMarq Cat# SPR-461)
Formulation :PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Stability :1yr at –200C from date of shipment. Avoid freeze/thaw cycle.
Scientific Background :Alzheimer’s Disease (AD) is the most common neurodegenerative disease, affecting 10% of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing paired helical filaments (PHFs). A truncated 95-amino acid fragment corresponding to residues 297-391 of full-length tau has been shown to assemble into PHF-like fibrils in vitro in the absence of additives or templates (3). This fragment has been found in the core of PHFs from AD brains and forms filaments that closely resemble PHFs isolated from AD brains (3).
References :1. www.alz.org/alzheimers-dementia/facts-figures
2. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146–148 (1907)
3. Al-Hilaly, Y.K. et al. Alzheimer's Disease-like Paired Helical Filament Assembly from Truncated Tau Protein Is Independent of Disulfide Crosslinking. J. Mol. Biol. 429(23):3650-3665 (2017)
Sample Data :Dot blot analysis using Rabbit Tau Polyclonal Antibody (Cat# T08-63R). Tissue: Recombinant Tau Monomers and Fibrils. Species: Human. Primary Antibody: Rabbit Tau Polyclonal Antibody (Cat# T08-63R) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. 
Sample Data :Western Blot Analysis of Mouse, Rat Brain showing detection of Multiple bands due to different isoforms/aggregation states of Tau protein using Rabbit Tau Polyclonal Antibody (Cat# T08-63R). Lane 1: MW Ladder. Lane 2: Mouse brain (15 µg). Lane 3: Rat brain (15 µg). Predicted/Observed Size: Multiple bands due to different isoforms/aggregation states. Other Band(s): 50 kDa, 65 kDa, 100 kDa. 
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