Tau Antibody, 1D5(T08-60M)
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Description :Mouse Monoclonal Antibody
Genbank Number :NM_020630
Cited Applications :WB (1:1000); DB; ICC/IF; ELISA
Ideal working dilutions for each application should be empirically determined by the investigator.
Specificity :Detects multiple bands. Antibody detects monomer under denaturing conditions but preferentially detects fibril under native conditions (dot blot).
Cross Reactivity :Human, Mouse, Rat
Host/Isotype/Clone# :Mouse / IgG1 / 1D5
Immunogen :Human Recombinant Tau441 (2N4R), P301S mutant Protein Pre-formed fibrils
Formulation :PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Stability :1yr at –200C from date of shipment. Avoid freeze/thaw cycle.
Scientific Background :Alzheimer’s Disease (AD) is the most common neurodegenerative disease, affecting 10% of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3). There are six isoforms of tau in the adult human brain: three with four repeat units (4R) and three with three repeat units (3R) (4). 2N4R, or Tau-441 is the full length tau protein. P301S is a mutation encoded by exon 10 (4) that impairs the ability of tau to assemble microtubules (5).
References :1. www.alz.org/alzheimers-dementia/facts-figures
2. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146–148 (1907)
3. Matsumoto, G. et al. (2018). Int J Mol Sci. 19, 1497.
4. Goedert, M. and Spillantini, M. G. (2017). Mol Brain. 10:18.
5. Bugiani, O. et al. (1999). J Neuropathol Exp Neurol. 58(6):667-77.
Sample Data :Dot Blot analysis using Mouse Tau Monoclonal Antibody, 1D5 (Cat# T08-60M). Tissue: Recombinant Protein. Species: Human. Primary Antibody: Mouse Tau Monoclonal Antibody, 1D5 (Cat# T08-60M) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking. 
Sample Data :Western Blot analysis of Human Breast Cancer Cell line with Mouse Brain showing detection of Tau protein using Mouse Tau Monoclonal Antibody, 1D5 (Cat# T08-60M). Lane1: MW Marker. Lane 2: Human T-47d (10 µg). Lane 3: Mouse Brain (20 µg). 
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