Stem Cell Factor (KITLG)(S816-40N)

Stem Cell Factor (KITLG)(S816-40N)

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Description :Recombinant Human Stem Cell Factor (KITLG) was expressed in E. coli cells.

Species :Human

Tag :

Expression System:E.coli

Sequence :

Genbank Number :P21583

Storage, Stability and Shipping :The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications :Cell Assay

Endotoxin Level :<1.0 EU/μg of recombinant protein as determined by the LAL method

Molecular Weight :18.7 kDa

Gene Aliases  :SCF, Kit ligand, Mast cell growth factor, MGF, c-Kit Ligand

Reconstitution Protocol :A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Scientific Background :SCF is a stromal cell-derived cytokine synthesized by fibroblasts and other cell types. SCF promotes proliferation and early differentiation of cells at the level of multipotent stem cells. It has been suggested that SCF is essential for optimal production of various hematopoietic lineages, mainly because of its ability to prevent apoptosis when co-stimulated with other cytokines. The receptor for SCF, designated SCFR (CD117) is the oncogene designated as KIT. The biological activities of SCF are considerably synergised by colony stimulating factors; GM-CSF and G-CSF, and by IL7, EPO and some other growth and differentiation factors. In combination with IL7, SCF stimulates the proliferation of pre-B cells. SCF is also a potent chemoattractant for cells expressing the kit receptor. Recombinant Human SCF is a monomeric protein that contains intra-chain disulfide bonds.

Sample Cell Assay Data :The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells was found to be ≤ 0.1 ng/Ml

Purity :Recombinant Human Stem Cell Factor (KITLG) resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250

Product Sheets (By Lot #) :

G3223-13.pdf

Research Areas :Cancer