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Description :Recombinant Streptavidin (aa 25-183) from Streptomyces avidinii was expressed in E. coli using N-terminal His tag.

Species :Streptomyces Avidinii

Tag :HIS

Expression System:E.coli

Sequence :aa 25-183

Genbank Number :X03591

Alternative name(s) :SA

Formulation :Recombinant protein stored in 50mM Na2HPO4, pH7.5, 300mM NaCl, 200mM imidazole, 1mM DTT, 10% glycerol.

Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background :Streptavidin (SA) is a tetrameric protein originating from the bacterium Streptomyces avidinii. Each subunit of the streptavidin tetramer can associate with one biotin small vitamin molecule. Although the SA-biotin interaction is non-covalent, it has an extremely high affinity (Kd ~10-14 M) (1). The SA-Biotin complex is highly thermostable and resistant to extreme pH, denaturing reagents, and enzyme degradation (2). By targeting biotin to biomolecules this interaction with SA can be exploited in a number of biochemical assays (3).

References :1. Weber PC, Cox MJ, Salemme FR, Ohlendorf DH. 1987. Crystallographic data for Streptomyces avidinii streptavidin. J Biol Chem.262(26):12728-9.
2. González M, Argaraña CE, Fidelio GD. 1999. Extremely high thermal stability of streptavidin and avidin upon biotin binding. Biomol Eng. 16(1-4):67-72.
3. Dundas CM, Demonte D, Park S. 2013. Streptavidin-biotin technology: improvements and innovations in chemical and biological applications. Appl Microbiol Biotechnol. 97(21):9343-53.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

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