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SRGAP3-BRAF (Sex12Bex9), Active(B08-19FG)

SRGAP3-BRAF (Sex12Bex9), Active(B08-19FG)

  • $259.00


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Description :Recombinant human fusion SRGAP3 (1-513 exon12)-BRAF (381-end or exon9-18) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :1-513 exon12 (SRGAP3) 381-end or exon 9-18 (BRAF)

Genbank Number :NM_014850

Genbank Number 2 :NM_004333

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay

Molecular Weight :~128 kDa

Gene Aliases :SRGAP3: ARHGAP14; MEGAP; SRGAP2; WRP
BRAF: BRAF1, RAFB1, B-raf, MGC126806, MGC138284

Scientific Background :KIAA1549-BRAF is a gene fusion resulting from a tandem duplication event involving the BRAF kinase gene that have recently been identified as the most frequent genetic alteration in many cancers. The KIAA1549-BRAF fusion typically results from a 2.0 Mb tandem duplication in chromosome band 7q34 (1). The KIAA1549:BRAF fusion gene is considered a driver genetic event in pilocytic astrocytoma and many other pediatric brain neoplasms. KIAA1549-BRAF fusion gene and BRAF (V600E) mutation may be responsible for deregulation of the Ras-RAF-ERK signaling pathway in many brain cancers (2).

References :
1. Dougherty, M J. et al: Activating mutations in BRAF characterize a spectrum of pediatric low-grade gliomas. Neuro Oncol. 2010 Jul;12(7):621-30.

2. Badiali, M. et al: KIAA1549-BRAF fusions and IDH mutations can coexist in diffuse gliomas of adults. Brain Pathol. 2012 Nov;22(6):841-7.

Product Sheets (By Lot #) :

K1685-3.pdf

W3329-11.pdf

Research Areas :Apoptosis/Autophagy, Angiogenesis, Cancer, JAK/STAT Pathway, Ser/Thr Kinases