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SMAD3 Protein(S12-30G)

SMAD3 Protein(S12-30G)

  • $215.00


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Description :Recombinant full-length human SMAD3 was expressed in E. coli cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:E.coli

Sequence :Full Length

Genbank Number :NM_005902

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~77 kDa

Gene Aliases :MADH3, JV15-2, HSPC193, HsT17436, MGC60396, DKFZP586N0721, DKFZp686J10186

Scientific Background :SMAD3 is a direct mediator of transcriptional activation by the TGFβ receptor. The activity of SMAD3 is regulated by the TGFβ receptors, and SMAD3 is phosphorylated and associated with the ligand-bound receptor complex. TGFβ stimulation leads to phosphorylation and activation of SMAD3, which form a complex with SMAD4 that accumulate in the nucleus and regulate transcription of target genes such as CDK inhibitor (1). SMAD3 containing a C-terminal truncation acts as a dominant-negative inhibitor of the normal TGFβ response. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2 (2).

References :
1. Inman, G. J. et al: Nucleocytoplasmic shuttling of Smads 2, 3, and 4 permits sensing of TGF-beta receptor activity. Molec. Cell 10: 283-294, 2002.

2. Matsuura, I. et al: Cyclin-dependent kinases regulate the antiproliferative function of Smads. Nature 430: 226-231, 2004.

Product Sheets (By Lot #) :

V201-4.pdf

W245-3.pdf

I3226-5.pdf

Research Areas :Cancer, Inflammation, AKT/PKB Pathway, ERK/MAPK Pathway, JNK/SAPK Pathway, NfkB Pathway, JAK/STAT Pathway, WNT Signaling, Cell Cycle, Cellular Stress, Angiogenesis, Cancer, Inflammation, AKT/PKB Pathway, ERK/MAPK Pathway, JNK/SAPK Pathway, NfkB Pathway, JAK/STAT Pathway, WNT Signaling, Cell Cycle, Cellular Stress, Angiogenesis