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Description :Recombinant full length human SHIP2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_001567
Specific Activity :Sample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Phosphatase Assay, Western Blot
Molecular Weight :175 kDa
Gene Aliases :INPPL1
Scientific Background :The SH2-containing inositol-5′-phosphatase 2 (SHIP2) belongs to the family of the mammalian inositol polyphosphate 5-phosphatases that catalyze the dephosphorylation of phosphoinositides and inositol phosphates with a phosphate at 5-position of the inositol ring. In addition to the N-terminal SH2 domains and N- and C-terminal proline-rich regions with consensus sites for SH3 domain interactions, SHIP2 also contains an SAM domain in the C-terminus interacting with the EphA2 receptor and with a PI 3-kinase effector protein regulating both Arf and Rho GTPases, ARAP3.
1. Pesesse X, et al: Identification of a second SH2-domain-containing protein closely related to the phosphatidylinositol polyphosphate 5-phosphatase SHIP. Biochem Biophys Res Commun. 239(3): 697-700, 1997.
2. Zhuang G, et al: Regulation of EphA2 receptor endocytosis by SHIP2 lipid phosphatase via phosphatidylinositol 3-Kinase-dependent Rac1 activation. J Biol Chem. 282(4): 2683-94, 2007.
3. Raaijmakers JH, et al: The PI3K effector Arap3 interacts with the PI (3,4,5) P3 phosphatase SHIP2 in a SAM domain-dependent manner. Cell Signal. 19(6): 1249-57, 2007.
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Research Areas :Lipid Kinases, Inflammation, Apoptosis/Autophagy, AKT/PKB Pathway