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SHH(S851-40N)

SHH(S851-40N)

  • $270.00


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Description :Recombinant Human SHH was expressed in E. coli cells.

Species :Human

Tag :

Expression System:E.coli

Sequence :

Genbank Number :Q15465

Storage, Stability and Shipping :The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications :Cell Assay

Endotoxin Level :<1.0 EU/μg of recombinant protein as determined by the LAL method

Molecular Weight :20.1 kDa

Gene Aliases :SHH, HHG-1, Recombinant Human Sonic Hedgehog (SHH)

Reconstitution Protocol :A quick spin of the vial followed by reconstitution at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Scientific Background :Highly conserved proteins, members of the Hedgehog family are widely represented throughout the animal kingdom. The three known mammalian Hedgehog proteins; Sonic (SHH), Desert (DHH) and Indian (IHH) hedgehog share a high degree of sequence and structural identity. SHH is specifically known to be expressed in fetal intestine, liver, lung and kidney. Recombinant Human SHH represents the N-product (N-terminal domain with Cys24 substituted by an Ile-Val-Ile sequence) which retains all the known signalling capabilities.

Sample Cell Assay Data :Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.1­ - 0.4 µg/mL.

Purity :Recombinant Human SHH resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250

Product Sheets (By Lot #) :

G3223-12.pdf

Research Areas :Cancer