SETDB1 (KMT1E) Protein(S346-31G)
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Description :Recombinant human SETDB1 (KMT1E) (715-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag.
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Genbank Number :BC028671
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Methyltransferase Assay
Molecular Weight :~100 kDa
Gene Aliases :KMT1E, ESET, KG1T, KIAA0067
Scientific Background :Histone-lysine N-methyltransferase SETDB1 (SETD6) specifically trimethylates 'Lys-9' of histone H3 and plays a central role in the silencing of euchromatic genes. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells by forming a corepressor complex. It was also found to be responsible for transcriptionally repressive state of genes in embryonic stem cells (ESCs).
1. Serra RW, et al: A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype. Elife. 2014 Mar 12;3:e02313.
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Research Areas :Apoptosis/Autophagy, Cancer, Cell Cycle, Neurobiology