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Description :Recombinant full-length human SETD7 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_030648
Specific Activity :Sample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Methyltransferase Assay
Molecular Weight :~75 kDa
Gene Aliases :FLJ21193; KIAA1717; KMT7; SET7; SET7/9; SET9
Scientific Background :SETD7 or SET domain containing lysine methyltransferase 7 is a lysine methyltransferase which can methylates lysine-4 (K4) in histone H3 in vitro and in vivo. Methylation of K4 in histone H3 by SETD7 and methylation of K9 in histone H3 by SUV39H1 were found to have differential effects on subsequent histone acetylation by p300 (1). SETD7 can also methylate p53 at lys372 within the C-terminal regulatory region (2). Methylated p53 is restricted to the nucleus and the modification positively affects its stability. SETD7 regulates the expression of p53 target genes in a manner dependent on the p53 methylation site.
1. Wang, H. et al: Purification and functional characterization of a histone H3-lysine 4-specific methyltransferase. Molec. Cell 8: 1207-1217, 2001.
2. Chuikov, S. et al: Regulation of p53 activity through lysine methylation. Nature 432: 353-360, 2004.
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Research Areas :Apoptosis/Autophagy, Cancer, Cell Cycle, Neurobiology, Cancer, Neurobiology, Apoptosis/Autophagy, Cell Cycle