Anti-SIRT2(S36-463BR)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the SIRT2 protein
Cited Applications :WB, ELISA, IF, IHC
Cross Reactivity :Human, Mouse and Rat
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :SIRT2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of the human SIRT2
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of SIRT2 in human brain lysate with SIRT2 antibody at (A) 2.5 and (B) 5 ug/ml.
Sample Data :Immunohistochemical staining of human brain tissue using SIRT2 antibody at 2.5 ug/ml.
Scientific Background :SIRT2 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT2 being a member of class I. Inhibition of SIRT2 can lead to neuroprotection in cellular and invertebrate models of Huntington's disease (1). Huntington's disease is characterized by increased sterol synthesis in neuronal cells and this process is reversed by SIRT2 inhibition. SIRT2 can deacetylate lys40 of alpha-tubulin both in vitro and in vivo (2). Knockdown of SIRT2 via small interfering RNA results in tubulin hyperacetylation.
References :
1. Luthi-Carter, R. Et al: SIRT2 inhibition achieves neuroprotection by decreasing sterol biosynthesis. Proc Natl Acad Sci U S A. 2010 Apr 27;107(17):7927-32.
2. North, B. J. et al: The human Sir2 ortholog, SIRT2, is an NAD(+)-dependent tubulin deacetylase. Molec. Cell 11: 437-444, 2003.
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Research Areas :Cancer, Cell Cycle, Inflammation, Cancer, Inflammation, Cell Cycle