Alpha Synuclein Antibody (pSer129), J18(S22-65R)
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Description :Rabbit Monoclonal Antibody
Genbank Number :NM_020630
Cited Applications :WB (1:500); ELISA; ICC; IHC
Ideal working dilutions for each application should be empirically determined by the investigator
Specificity :Binds to phosphorylated serine 129 on alpha synuclein. Does not detect unphosphorylated serine 129 alpha synuclein.
Cross Reactivity :Human, Mouse
Host/Isotype/Clone# :Rabbit / IgG / J18
Immunogen :Human alpha synuclein AA 124-134: AYEMP-pS-EEGYQ-Cys
Formulation :PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Stability :1yr at –200C from date of shipment. Avoid freeze/thaw cycle.
Scientific Background :Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson’s disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6). Alpha synuclein phosphorylated at serine 129 constitutes 90% of the alpha synuclein found in Lewy bodies (7, 8).
References :1. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013).
2. Zhang L., et al. (2008) Brain Res. 1244: 40-52.
3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117.
4. Kokhan V.S., Afanasyeva M.A., Van’kin G. (2012) Behav. Brain. Res. 231(1): 226-230.
5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840.
6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757.
7. Fujiwara H., et al. (2002) Nat Cell Biol. 4(2):160-4.
8. Anderson J.P., et al. (2006) J Biol Chem. 281(40):29739-52.
Sample Data :Western Blot analysis of Human Alpha Synuclein showing detection of Alpha Synuclein pSer129 protein using Rabbit Alpha synuclein Antibody (pSer129), J18 (Cat# S22-65R). Lane 1: MW ladder. Lane 2: 0.5 µg human alpha synuclein monomer (Cat# S22-371N). Lane 3: 2 µg human alpha synuclein monomer (Cat# S22-371N). Lane 4: 0.5 µg human alpha synuclein PFFs (Cat# S22-381N). Lane 5: 2 µg human alpha synuclein PFFs (Cat# S22-381N). Lane 6: 15 µg human Parkinson’s Disease brain lysate. 
Sample Data :Western Blot analysis of Mouse Brain showing detection of Alpha Synuclein pSer129 protein using Rabbit Alpha Synuclein Antibody (pSer129), J18 (Cat# S22-65R). Lane 1: MW ladder. Lane 2: Mouse brain. 
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