Alpha Synuclein Antibody, 3C11(S22-61M)
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Description :Mouse Monoclonal Antibody
Genbank Number :NM_020630
Cited Applications :WB (1:1000); DB (1:1000); ICC/IF (1:100); ELISA (1:1000)
Ideal working dilutions for each application should be empirically determined by the investigator.
Specificity :Binds with the 81-130 aa region of human ASYN, determined using WB and ELISA with corresponding peptides. Detects ~14kDa band corresponding to ASYN. Band at ~30kDa is a dimer.
Cross Reactivity :Human, Mouse, Rat
Cross Reactivity :Human, Mouse, Rat
Host/Isotype/Clone# :Mouse / IgG1 / 3C11
Immunogen :Human alpha synuclein monomer
Formulation :PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Stability :1yr at –200C from date of shipment. Avoid freeze/thaw cycle.
Scientific Background :Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6).
References :1. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013).
2. Zhang L., et al. (2008) Brain Res. 1244: 40-52.
3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117.
4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230.
5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840.
6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757.
Sample Data :Western Blot analysis of Human Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Alpha Synuclein Antibody, 3C11 (Cat# S22-61M). Lane 1: Molecular weight ladder (MW). Lane 2: Parkinson brain cell lysate. Lane 3. Human brain cell lysate. Predicted/Observed Size: 14 kDa. Other Band(s): 100 kDa (oligomer).
Sample Data :Western Blot analysis of Mouse, Rat Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Alpha Synuclein Antibody, 3C11 (Cat# S22-61M). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain cell lysate. Lane 3: Rat brain cell lysate. Predicted/Observed Size: 14 kDa. Other Band(s): ~30 kDa (dimer).
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