Alpha Synuclein Antibody, 10H7(S22-61BM)

Alpha Synuclein Antibody, 10H7(S22-61BM)

  • $415.00


Description :Mouse Monoclonal Antibody

Genbank Number :NM_020630

Cited Applications :WB (1:1000); DB (1:1000); ICC/IF (1:100); ELISA (1:1000)

Ideal working dilutions for each application should be empirically determined by the investigator.

Specificity :Binds with the 101-130 aa region of human ASYN, determined using DB and ELISA with corresponding peptides. Detects ~14kDa band corresponding to ASYN. ~40 kDa is a trimer. Does not detect truncated ASYN (aa 1-114).

Cross Reactivity :Human, Mouse, Rat

Host/Isotype/Clone# :Mouse / IgG1 / 10H7

Immunogen :Mouse alpha synuclein aggregate

Formulation :PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.

Stability :1yr at –200C from date of shipment. Avoid freeze/thaw cycle.

Scientific Background :Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6).

References :1. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013).
2. Zhang L., et al. (2008) Brain Res. 1244: 40-52.
3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117.
4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230.
5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840.
6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757.

Sample Data :Western Blot analysis of Human, Mouse, Rat Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Alpha Synuclein Antibody, 10H7 (Cat# S22-61BM). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain cell lysate. Lane 3: Rat brain cell lysate. Lane 4: Human brain cell lysate. Predicted/Observed Size: 14 kDa. Other Band(s): ~40 kDa (trimer)

Sample Data :Immunocytochemistry/immunofluorescence analysis using Mouse Alpha Synuclein Antibody, 10H7 (Cat# S22-61BM). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.

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