Anti-SIK1(S14-63R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the SIK1 protein
Cited Applications :WB, ELISA, IHC
Cross Reactivity :Human
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :SIK1 antibody was raised against an 18 amino acid synthetic peptide near the center of human SIK1
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of SIK1 in human small intestine tissue lysate with SIK1 antibody at 1 ug/ml.
Sample Data :Immunohistochemistry of SIK1 in human small intestine tissue with SIK1 antibody at 5 ug/ml.
Scientific Background :SIK is a protein kinase that is involved in regulating AMPK-related kinases (1). SIK may mediate the physiological effects of LKB1, including its tumour suppressor function. SIK is also involved in signaling by various proteins like STRAD, NUAK1, NUAK2, BRSK1, BRSK2, QIK, QSK, SIK, MARK1, MARK2, MARK3, MARK4 and MELK that are related to AMPK. Activation of SIK1 by phosphorylation on thr322 can lead to an increase in the catalytic activity of sodium/potassium ATPase alpha subunit at the plasma membrane (2). This results in an increase in intracellular sodium in intact mammalian cells.
References :
1. Takemori, H. Et al: TORC-SIK cascade regulates CREB activity through the basic leucine zipper domain. FEBS J. 2007; 274 (13): 3202–9.
2. Sjostrom, M.; SIK1 is part of a cell sodium-sensing network that regulates active sodium transport through a calcium-dependent process. Proc. Nat. Acad. Sci. 104: 16922-16927, 2007.
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Research Areas :Cancer, Ser/Thr Kinases, Cancer, Ser/Thr Kinases