SIK, Active(S14-10G)

SIK, Active(S14-10G)

  • $435.00


FOR BULK ORDER REQUESTS PLEASE CONTACT US

Description :Recombinant full-length human SIK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST

Expression System:Sf9 insect cells using baculovirus

Sequence :Full Length

Genbank Number :NM_173354

Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Alternative Name(s) :SNF1LK, MSK, SIK1

Formulation :Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, and 25% glycerol.

Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background :SIK is a protein kinase that is involved in regulating AMPK-related kinases (1). SIK may mediate the physiological effects of LKB1, including its tumour suppressor function. SIK is also involved in signaling by various proteins like STRAD, NUAK1, NUAK2, BRSK1, BRSK2, QIK, QSK, SIK, MARK1, MARK2, MARK3, MARK4 and MELK that are related to AMPK. Activation of SIK by phosphorylation on thr322 can lead to an increase in the catalytic activity of sodium/potassium ATPase alpha subunit at the plasma membrane (2). This results in an increase in intracellular sodium in intact mammalian cells.

References :1. Takemori, H. Et al: TORC-SIK cascade regulates CREB activity through the basic leucine zipper domain. FEBS J. 2007; 274 (13): 3202–9.
2. Sjostrom, M.; SIK is part of a cell sodium-sensing network that regulates active sodium transport through a calcium-dependent process. Proc. Nat. Acad. Sci. 104: 16922-16927, 2007.

Molecular Weight :Observed MW ~120 kDa. Calculated MW ~115 kDa.

Product Sheets (By Lot #) :

U4803-1.pdf

Research Areas :Cell Cycle, PKA/PKC Pathway