Anti-SIK3(S12-63R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the SIK3 protein
Cited Applications :WB, ELISA, IF
Cross Reactivity :Human, Mouse and Rat
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :SIK3 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SIK3
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of SIK3 in rat brain tissue lysate with SIK3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Sample Data :Immunofluorescence of SIK3 in rat brain cells with SIK3 antibody at 20 ug/ml.
Scientific Background :SIK3 (QSK) is a serine/threonine-protein kinase, belongs to QIK subfamily. The phosphorylation of SIK3 by LKB1 through the 14-3-3 binding enhances its catalytic activity and leads its localization to punctate structures within the cytoplasm (1). Overexpression of SIK3 promotes G1/S cell cycle progression with ovarian cancer (2). There are two sites (H331L and A1103V) were mutated at significant frequency in breast cancer (3). SIK3 is a novel tumor-associated antigen (TAA).
References :
1. Al-Hakim A.K., et.al: 14-3-3 cooperates with LKB1 to regulate the activity and localization of QSK and SIK. J. Cell Sci. 118:5661-5673(2005).
2. Charoenfuprasert S, et.al: Identification of salt-inducible kinase 3 as a novel tumor antigen associated with tumorigenesis of ovarian cancer. Oncogene. 2011 Aug 18;30(33):3570-84.
3. Sjoeblom T. et.al: The consensus coding sequences of human breast and colorectal cancers. Science 314:268-274(2006).
Product Sheets (By Lot #) :
Research Areas :Cancer, Ser/Thr Kinases, Cancer, Ser/Thr Kinases