Anti-SMAD2(S11-363R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the human SMAD2 protein
Cited Applications :ELISA, IP, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human, Mouse and Rat
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against synthesized peptide corresponding to an internal region of human SMAD2 protein.
Purification :Affinity chromatography
Stability :1yr at –20oC from date of shipment
Sample Data :Western blot using affinity purified Anti-SMAD2 (1:2,500 dilution) to detect over-expressed Smad2 in COS cells (arrow). Lane C shows mock infection of COS cells with lentiviral vector alone. Lane S2 shows detection of Smad2 in lysates of COS transfected with Smad2. Lane V contains lysates of MDA-MB231 cells treated with vehicle; the next lane contains lysates of MDA-MB231 cells treated with TGFβ.
Scientific Background :SMADs are essential intracellular components for the signal transduction of TGFβ family members. SMAD2 is an intracellular mediator of TGFβ family and activin type 1 receptor (1). SMAD2 mediate TGFβ signaling to regulate cell growth and differentiation. SMAD2 is released from cytoplasmic retention by TGFβ receptor-mediated phosphorylation. The phosphorylated SMAD2 then forms a heterodimeric complex with SMAD4, and this complex translocates from cytoplasm into nucleus. By interacting with DNA-binding proteins, SMAD2 complexes then positively or negatively regulate the transcription of target genes. Inactivating mutations in SMAD2 have been found in various cancers (2).
References :
1. Masayuki, F. et al: Identification and Characterization of Constitutively Active Smad2 Mutants: Evaluation of Formation of Smad Complex and Subcellular Distribution. Molecular Endocrinol. 2000; 14 (10): 1583-1591.
2. Eppert, K. et al: MADR2 maps to 18q21 and encodes a TGF-beta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma. Cell. 1996; 86: 543-552.
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Research Areas :AKT/PKB Pathway, Angiogenesis, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Inflammation, JAK/STAT Pathway, JNK/SAPK Pathway, NfkB Pathway, WNT Signaling